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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be observed in lots of tumors, which leads to chromosome instability, and therefore could lead to tumorigenesis. This protein was observed to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important role in marketing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be described. Two extra splice variants were reported but comprehensive nucleotide sequence info is not yet readily available.

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Blocking peptides are peptides that bind particularly into the target antibody and block antibody binding. These peptide commonly contains the epitope acknowledged via the antibody.

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This guideline outlines even further doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This manual outlines even more feasible optimization of mobile and immunoassay parameters to be sure the very best final results are...

Antibodies sure to the blocking peptide no longer bind to your epitope to the concentrate on protein. By comparing the staining with the blocked antibody vs . the antibody alone, you can see which staining is certain.

Goal Facts Cyclin E belongs on the remarkably conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit unique expression and degradation styles which lead towards the temporal coordination of each mitotic party. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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